Growth Profiles, Nutrient composition and Pigments Analysis of Dunaliella salina strain San Quintin

Ricardo Valencia, Ivone Giffard-Mena, Ricardo Cruz-López, Ernesto García-Mendoza, José Luis Stephano-Hornedo

Abstract


Some microalgae are recognized for producing pigments and other metabolites with biotechnological importance, particularly, <i>Dunaliella salina</i> is a remarkable one. These kind of compounds are used as food and have a great industrial potential. The pigment industry comprises a millionaire market value, being β-carotene one of the most profitable one. In this study we describe the morphology, molecular identification, growth dynamics, proximal composition, nutrients and pigment content of a recently isolated <i>Dunaliella salina</i> strain (SQ) under different salinity/light conditions, in order to highlight its remarkable properties for biotech/biomed industry. <i>D. salina</i> SQ reached the highest densities (1.07-1.25 cell mL-1 x106) at low salinities (100-500 mM NaCl) under continuous light regimen (24:0 h Light:Dark). Neoxanthin (Neo) and violaxanthin (Viol) were the most abundant pigments when exposed to 500 mM NaCl (18:6 h Light:Dark). Furthermore, this peculiar strain produces other compounds with high industrial value.

Perfiles de crecimiento, composición de nutrientes y análisis de pigmentos de <i>Dunaliella salina</i> cepa San Quintín

 

 

Algunas microalgas son reconocidas por producir pigmentos y otros metabolitos con importancia biotecnológica, en particular, <i>Dunaliella salina</i> es una de las más notables. Este tipo de compuestos se usan como alimento y tienen potencial industrial. La industria del pigmento tiene un valor de mercado millonario, siendo el β-caroteno uno de los más rentables. En este estudio se describe la morfología, la dinámica de crecimiento, composición proximal, composición de nutrientes y contenido de pigmentos de una cepa de <i>Dunaliella salina</i> (SQ) recientemente aislada. La identificación de la especie se corroboró mediante técnicas moleculares. Se cultivó a <i>D. salina</i> bajo diferentes condiciones de salinidad y luz, con el objetivo de resaltar sus propiedades para la industria biotecnológica y biomédica. <i>D. salina</i> SQ alcanzó las densidades más altas (1.07-1.25 células mL-1 x106) a salinidades bajas (NaCl 100 y 500 mM) en un régimen de luz continua. La neoxantina (Neo) y la violaxantina (Viol) fueron los pigmentos más abundantes en 500 mM NaCl y un ciclo de luz: oscuridad 18: 6 h. Además, esta peculiar cepa produce otros compuestos con alto valor industrial.


Keywords


Dunaliella salina; green microalgae; pigments; sea salt fields; salinity challenge

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DOI: http://dx.doi.org/10.37543/oceanides.v33i2.212

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